Light Fluorescent
Subnanometre single-molecule localization, registration and distance measurements
Remarkable progress in optical microscopy has been made in the measurement of nanometre distances. If diffraction blurs the image of a point object into an Airy disk with a root-mean-squared (r.m.s.) size of s = 0.44λ/2NA (∼90 nm for light with a wavelength of λ = 600 nm and an objective lens with a numerical aperture of NA = 1.49), limiting the resolution of the far-field microscope in use to d ...

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Light Fluorescent

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